Choose the correct sequence of steps involved in the produc…

Biology Biotechnology and Its Application Statement Based Questions Single Correct MCQ

Published by: CGP EDU Academic Team

Published on: August 20, 2026

Choose the correct sequence of steps involved in the production of humulin.

(I) Synthesis of gene (DNA) for human insulin artificially

(II) Recombinant E. coli culture in bioreactor

(III) Humulin purification

(IV) Insertion of human insulin gene into plasmid

(V) Recombinant plasmid introduction into E. coli

(VI) Extraction of recombinant gene product from E. coli

(II) → (I) → (IV) → (III) → (V) → (VI)

(I) → (III) → (V) → (VI) → (II) → (IV)

(I) → (IV) → (V) → (II) → (VI) → (III)

(III) → (V) → (II) → (I) → (VI) → (IV)

Text Solution

Verified by Experts

The correct answer is:

(I): Artificial synthesis of insulin gene

(IV): Insertion into plasmid

(V): Recombinant plasmid introduced into E. coli

(II): Culture of recombinant E. coli in bioreactor

(VI): Extraction of insulin product

(III): Purification of Humulin

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